| Gene | Ffar1 |
| Chromosome | 7 |
| Deletion Start | 30,861,467 |
| Deletion End | 30,860,526 |
| Deletion Size | 942 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,861,335 | 30,861,354 | TCCCAGCTTGGTCTACACTC |
| TUP | 30,861,311 | 30,861,334 | TCCATCTGGGCTGCTCTGATCTCC |
| TUR | 30,861,271 | 30,861,290 | AGGGCCTCCACAGCCTTCAG |
| TDF | 30,860,898 | 30,860,921 | CTCAGTTTCTCCATTCTGCTCTTC |
| TDP | 30,860,874 | 30,860,896 | TTCTGCCCTTGGTCATCACTGCC |
| TDR | 30,860,813 | 30,860,831 | GCTTCCGTTTGTGGCTCAG |
PCR Primers
| Primer | Sequence |
| SU | CCCGAGTTCGGTTGCTCTC |
| SD | CAGAAGCAGTTGGGAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 299 bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | 305 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10614C-F6 | VGB6 | C57BL/6NTac | |
| 10614A-D4 | VGB6 | C57BL/6NTac | |
