| Gene | Islr |
| Chromosome | 9 |
| Deletion Start | 58,158,219 |
| Deletion End | 58,156,918 |
| Deletion Size | 1,302 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,157,677 | 58,157,699 | TGCAGATCAATGACAACCCTTTC |
| TUP | 58,157,653 | 58,157,675 | ACTGCACCTGTGGCATCGTGTGG |
| TUR | 58,157,610 | 58,157,632 | GTTCTGGAATAGACACCGCTGAG |
| TDF | 58,157,260 | 58,157,279 | CTGACTTTGGCAAGCTGGAG |
| TDP | 58,157,236 | 58,157,258 | AGGGCACCTATAGCTGCCTGGCC |
| TDR | 58,157,215 | 58,157,233 | GGCACTGCCTAGCTCATTG |
PCR Primers
| Primer | Sequence |
| SU | CACACGACCTTGGCAAGTC |
| SD | TAGCTGCTAGTTCTGAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 333 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | 296 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10617A-B12 | VGB6 | C57BL/6NTac | |
