| Gene | Zfp352 |
| Chromosome | 4 |
| Deletion Start | 90,223,634 |
| Deletion End | 90,225,458 |
| Deletion Size | 1,825 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,223,663 | 90,223,683 | ACCCTGCAATACACAGCCTTC |
| TUP | 90,223,684 | 90,223,706 | CCCTGTGGAGTCCAGCCAGCTTT |
| TUR | 90,223,748 | 90,223,768 | ACAGGATGCAGATTCCATCAC |
| TDF | 90,224,621 | 90,224,644 | GATACAGCTCTTCCTGGGTCTAAG |
| TDP | 90,224,653 | 90,224,676 | ACCCAGTGCAGAAGACAACCTGGC |
| TDR | 90,224,689 | 90,224,710 | TTCTTCAGATGTGGGACTTGTC |
PCR Primers
| Primer | Sequence |
| SU | GTGCTAGGTTTTGTTCGTCC |
| SD | GAAAGATACCAAGAATCAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 376 bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | 305 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10619C-H9 | VGB6 | C57BL/6NTac | |
| 10619C-D1 | VGB6 | C57BL/6NTac | |
| 10619C-D2 | VGB6 | C57BL/6NTac | |
| 10619C-D7 | VGB6 | C57BL/6NTac | |
| 10619C-G4 | VGB6 | C57BL/6NTac | |
| 10619C-H1 | VGB6 | C57BL/6NTac | |
