| Gene | Mrgpre |
| Chromosome | 7 |
| Deletion Start | 143,781,761 |
| Deletion End | 143,780,551 |
| Deletion Size | 1,211 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 143,781,561 | 143,781,579 | CTGCGCCGACCTCATCTTC |
| TUP | 143,781,538 | 143,781,559 | TCTGCTGCCACATGGTGGCCAT |
| TUR | 143,781,439 | 143,781,459 | AGGCTCAGGCCCACAATGTAG |
| TDF | 143,781,090 | 143,781,109 | TCCTGCTCTTCCTCTTCTGC |
| TDP | 143,781,065 | 143,781,087 | CCTGCCCTTTGGCATCTTCTGGC |
| TDR | 143,781,041 | 143,781,060 | TGTGCCAGGACAGGTTCTTG |
PCR Primers
| Primer | Sequence |
| SU | GGCTCTTTGAACAACTGACAG |
| SD | TTCTGCCACTTAGGACTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 389 bp | - | + | + |
| TUF | TUR | 141 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 320 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10621A-H6 | VGB6 | C57BL/6NTac | |
| 10621A-E12 | VGB6 | C57BL/6NTac | |
| 10621B-B2 | VGB6 | C57BL/6NTac | |
| 10621B-C3 | VGB6 | C57BL/6NTac | |
| 10621B-G3 | VGB6 | C57BL/6NTac | |
| 10621B-E7 | VGB6 | C57BL/6NTac | |
| 10621B-E4 | VGB6 | C57BL/6NTac | |
| 10621B-E1 | VGB6 | C57BL/6NTac | |
