| Gene | Ogfr |
| Chromosome | 2 |
| Deletion Start | 180,589,465 |
| Deletion End | 180,595,701 |
| Deletion Size | 6,237 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 180,589,995 | 180,590,015 | CAAGCCGCATGTAAACCTCAG |
| TUP | 180,590,016 | 180,590,039 | TGGCGTTCTGTAAGACGCACTGGC |
| TUR | 180,590,052 | 180,590,072 | CTCCATCACACCCGGAATCAC |
| TDF | 180,594,934 | 180,594,954 | TGATGGAGTAGCCAGTAACAC |
| TDP | 180,594,956 | 180,594,977 | CAAGTGCAGGCCAGTGCCCTGT |
| TDR | 180,594,986 | 180,595,006 | GGACTCAGGACACTCTGAAGG |
PCR Primers
| Primer | Sequence |
| SU | CTCCAGGGTTGTCCTTCTG |
| SD | GGACAAAAGAGAAGGCTAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 326 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 269 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10622B-A7 | VGB6 | C57BL/6NTac | |
| 10622B-A9 | VGB6 | C57BL/6NTac | |
| 10622B-B5 | VGB6 | C57BL/6NTac | |
| 10622B-H5 | VGB6 | C57BL/6NTac | |
| 10622B-B10 | VGB6 | C57BL/6NTac | |
| 10622B-A12 | VGB6 | C57BL/6NTac | |
