| Gene | Rnf43 |
| Chromosome | 11 |
| Deletion Start | 87,727,139 |
| Deletion End | 87,734,329 |
| Deletion Size | 7,191 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,727,266 | 87,727,286 | TGGAGGAAGGTTGGGTTCTTG |
| TUP | 87,727,287 | 87,727,312 | AGCTGGTAGCAGAAGTCAAACAGCCC |
| TUR | 87,727,382 | 87,727,402 | TCAGCTTCGCAGCATCACTAC |
| TDF | 87,733,500 | 87,733,521 | CCACAGGCAGGAGAAACCTAAC |
| TDP | 87,733,523 | 87,733,545 | TGCTGTGGCCTTCAGTGCTCCAG |
| TDR | 87,733,575 | 87,733,595 | CAAAGCAGAGGGCAGAGGAAG |
PCR Primers
| Primer | Sequence |
| SU | CCAGTGAACCCTGGGTGAG |
| SD | CTTGTGTGAGCTTGAAGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 373 bp | - | + | + |
| TUF | TUR | 137 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | 373 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10623A-H3 | VGB6 | C57BL/6NTac | |
| 10623A-C11 | VGB6 | C57BL/6NTac | |
| 10623A-C2 | VGB6 | C57BL/6NTac | |
| 10623A-H2 | VGB6 | C57BL/6NTac | |
| 10623A-D3 | VGB6 | C57BL/6NTac | |
| 10623A-H7 | VGB6 | C57BL/6NTac | |
