| Gene | Fndc7 |
| Chromosome | 3 |
| Deletion Start | 108,889,703 |
| Deletion End | 108,856,345 |
| Deletion Size | 33,359 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,888,826 | 108,888,846 | GCCAGATAGCTGTGGACAAAC |
| TUP | 108,888,800 | 108,888,824 | AACACACGACCATCATTCCAGCCAA |
| TUR | 108,888,737 | 108,888,761 | AGGTCCCAAAAGATGAGTAGGAAAG |
| TDF | 108,856,438 | 108,856,457 | CAGCCTCTGTGTCCTCTTCC |
| TDP | 108,856,409 | 108,856,436 | TGTCCCTTTCTTCCCTGTCCCTTTAGTG |
| TDR | 108,856,376 | 108,856,397 | GCTCGCTGTATCGTTCCTCTTC |
PCR Primers
| Primer | Sequence |
| SU | CAGTCTTTGCAGTACCTGTC |
| SD | TGCAGCCAACGACACACTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 326 bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 322 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10627A-E2 | VGB6 | C57BL/6NTac | |
| 10627A-H3 | VGB6 | C57BL/6NTac | |
| 10627A-B7 | VGB6 | C57BL/6NTac | |
| 10627A-C11 | VGB6 | C57BL/6NTac | |
