| Gene | Klk4 |
| Chromosome | 7 |
| Deletion Start | 43,881,652 |
| Deletion End | 43,885,566 |
| Deletion Size | 3,915 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,882,412 | 43,882,433 | TGACCCGTCTTCCTGACAAATG |
| TUP | 43,882,435 | 43,882,463 | CCCTACATCCAGTGTGCTGATGATCCTGG |
| TUR | 43,882,499 | 43,882,520 | GGGTGGAGTTAGGTCCCTTGAG |
| TDF | 43,884,644 | 43,884,664 | GAGGATCAAGGAGGCAGAGAC |
| TDP | 43,884,666 | 43,884,692 | CAGAAGCCAGGCATAGTGGTTCAAACC |
| TDR | 43,884,705 | 43,884,725 | CACTGTGGGAGGGACTGAATG |
PCR Primers
| Primer | Sequence |
| SU | GATCTATGCCTCCATTTCTCC |
| SD | ACAGGCATAAGGTGTTTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 361 bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 296 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10628A-A3 | VGB6 | C57BL/6NTac | |
| 10628A-C1 | VGB6 | C57BL/6NTac | |
| 10628A-D4 | VGB6 | C57BL/6NTac | |
| 10628A-E6 | VGB6 | C57BL/6NTac | |
| 10628A-F2 | VGB6 | C57BL/6NTac | |
