| Gene | Bst2 |
| Chromosome | 8 |
| Deletion Start | 71,537,424 |
| Deletion End | 71,534,668 |
| Deletion Size | 2,757 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,536,999 | 71,537,021 | GAACTCCCTACAGCTCTGCAATC |
| TUP | 71,536,966 | 71,536,993 | CCTCAGTATCCTCCCTTCCTTTGGTGAC |
| TUR | 71,536,929 | 71,536,951 | GCTCTCCAACTCAAGCACTCTAC |
| TDF | 71,535,144 | 71,535,168 | GAATACAAATCAGAGCAGGCAGAAG |
| TDP | 71,535,121 | 71,535,143 | AGGCGACTCAGTGCCTAGAGCAC |
| TDR | 71,535,066 | 71,535,084 | GAGGTATGCACGTCGGTTC |
PCR Primers
| Primer | Sequence |
| SU | GCGAAGGCCAAGAATTTGAG |
| SD | ACTTCAAACAACTGTGACCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 352 bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | 306 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10630D-E5 | VGB6 | C57BL/6NTac | |
| 10630D-H2 | VGB6 | C57BL/6NTac | |
| 10630D-G11 | VGB6 | C57BL/6NTac | |
| 10630D-E12 | VGB6 | C57BL/6NTac | |
