10636   Tssk3     Status: ES cell colonies screened / QC positives

GeneTssk3
Chromosome4
Deletion Start129,490,603
Deletion End129,489,068
Deletion Size1,555
Genome Build38
MGI IDMGI:1929914
NCBI Gene ID58864
BAC ID332L14
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 4

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF129,490,406129,490,425CAACACGGGTAGGCCCTTTC
TUP129,490,376129,490,403CAGAGCATCTCTTCCTCCCTTTGAGTCA
TUR129,490,351129,490,374GAATCTGACTTGCTTTCTGCCATC
TDF129,489,352129,489,370TGTGGCAGCACAGCCTATG
TDP129,489,323129,489,346CCTGAGGTGCTACAGGGCATACCC
TDR129,489,290129,489,311CATGCTCCAGACATCACCTTTC

PCR Primers

PrimerSequence
SUGACACTGCCCATGTTGTCTG
SDCCTTAGTTCCAGGAAGGTCT
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev366 bp-++
TUFTUR75 bp++-
TDFTDR81 bp++-
NeoFwdSD281 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10636C-C10VGB6C57BL/6NTac
10636C-D6VGB6C57BL/6NTac
10636C-F2VGB6C57BL/6NTac
10636C-F5VGB6C57BL/6NTac
10636C-H10VGB6C57BL/6NTac
10636D-B6VGB6C57BL/6NTac
10636D-F3VGB6C57BL/6NTac
10636D-G8VGB6C57BL/6NTac
10636D-H6VGB6C57BL/6NTac
10636D-H8VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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