| Gene | Opn1sw |
| Chromosome | 6 |
| Deletion Start | 29,380,412 |
| Deletion End | 29,377,915 |
| Deletion Size | 2,498 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,380,115 | 29,380,138 | GCTGTCACGGATACTTCCTCTTTG |
| TUP | 29,380,090 | 29,380,112 | CGCCATGTTTGTGCTCTGGAGGC |
| TUR | 29,380,049 | 29,380,066 | AGCCCAGGTTGCCTTCAG |
| TDF | 29,378,238 | 29,378,260 | CAGGCTGTCCTTGAACTCAAGAG |
| TDP | 29,378,213 | 29,378,236 | TGGTCCTGCCTCCTATCCCGAGTG |
| TDR | 29,378,175 | 29,378,197 | GCTGGACTTATGTGCTGGTACAC |
PCR Primers
| Primer | Sequence |
| SU | CACAGAAACCAATACTGACC |
| SD | AAAGGAAGTGTTACCCATAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 416 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 333 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10640A-F7 | VGB6 | C57BL/6NTac | |
| 10640A-H6 | VGB6 | C57BL/6NTac | |
| 10640A-H9 | VGB6 | C57BL/6NTac | |
