| Gene | Zbtb5 |
| Chromosome | 4 |
| Deletion Start | 44,995,379 |
| Deletion End | 44,993,350 |
| Deletion Size | 2,030 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,994,554 | 44,994,572 | CAAGTGCCCAGCCAGTCAG |
| TUP | 44,994,530 | 44,994,553 | ATGGCAGTGCTGGCAACATGGCCT |
| TUR | 44,994,482 | 44,994,500 | CAGCCACGGCCTCTTGTTC |
| TDF | 44,994,099 | 44,994,120 | CACTGACGACAACCTCCCAAAC |
| TDP | 44,994,073 | 44,994,098 | ACCACCAGTGACTGTAGGCTCGAGGG |
| TDR | 44,994,039 | 44,994,057 | CCCAGCCTCTGGACTCAAC |
PCR Primers
| Primer | Sequence |
| SU | AAGGCTTTACCTCCATGGCC |
| SD | AAATGTTTTCTGCACTTTCA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 233 bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 330 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10644A-C11 | VGB6 | C57BL/6NTac | |
| 10644A-D2 | VGB6 | C57BL/6NTac | |
| 10644A-D4 | VGB6 | C57BL/6NTac | |
| 10644A-E12 | VGB6 | C57BL/6NTac | |
| 10644A-D11 | VGB6 | C57BL/6NTac | |
