| Gene | Gpr75 |
| Chromosome | 11 |
| Deletion Start | 30,891,100 |
| Deletion End | 30,892,719 |
| Deletion Size | 1,620 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,891,579 | 30,891,600 | CTTGCTCCTTACCCTTCTTCTC |
| TUP | 30,891,601 | 30,891,623 | TGGGCGACCAGCTTTACACTTGC |
| TUR | 30,891,639 | 30,891,662 | CACAGGTGGGACTTATTGGTTCTC |
| TDF | 30,892,454 | 30,892,474 | AAAGTGCAGCGAGTCCCAAAG |
| TDP | 30,892,477 | 30,892,501 | TCTGCTGGACATCAACCCTGTGGTC |
| TDR | 30,892,506 | 30,892,527 | AGTGTTGATGGGTGTGGAACTG |
PCR Primers
| Primer | Sequence |
| SU | TTTTCAAAGCAATAGAAATG |
| SD | AGTATTGGGACATAGATTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 372 bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 319 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10647A-A12 | VGB6 | C57BL/6NTac | |
| 10647A-C5 | VGB6 | C57BL/6NTac | |
| 10647A-F11 | VGB6 | C57BL/6NTac | |
| 10647A-H10 | VGB6 | C57BL/6NTac | |
