| Gene | Zhx3 |
| Chromosome | 2 |
| Deletion Start | 160,782,242 |
| Deletion End | 160,771,402 |
| Deletion Size | 10,841 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 160,781,795 | 160,781,819 | ACCAAGCCAGACAATCATGTAGTAG |
| TUP | 160,781,765 | 160,781,791 | AGCAGAGTGTCCCTGACAGTGCTAGCT |
| TUR | 160,781,730 | 160,781,750 | CCCTTCCGTAGTAGACTCACC |
| TDF | 160,771,847 | 160,771,869 | GTGAGTGACAGGAGCCTAAGATG |
| TDP | 160,771,818 | 160,771,841 | AGGCCTTGTCCAACTGAGCCAGCA |
| TDR | 160,771,772 | 160,771,792 | CAGCCTGAGAGGAGGTGAAAG |
PCR Primers
| Primer | Sequence |
| SU | AGCTCCTTGTGAACACTCAC |
| SD | CCACTGTTGCAGAGGATGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 368 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | 336 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10648A-G3 | VGB6 | C57BL/6NTac | |
| 10648C-B11 | VGB6 | C57BL/6NTac | |
| 10648C-H8 | VGB6 | C57BL/6NTac | |
