| Gene | Sox7 |
| Chromosome | 14 |
| Deletion Start | 63,943,740 |
| Deletion End | 63,948,888 |
| Deletion Size | 5,149 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 63,944,564 | 63,944,588 | GGTCTTTGAATCCCATCGCTTCTTG |
| TUP | 63,944,593 | 63,944,620 | TAGCTATGGTGCTGTCCGCAGATAATCC |
| TUR | 63,944,631 | 63,944,651 | CCCAATTCTCTGGCCTGTCTG |
| TDF | 63,948,711 | 63,948,731 | AGCACTGAGCTGAGCAGAAAC |
| TDP | 63,948,734 | 63,948,755 | TGGCGATGCCACTCCTTGGTGG |
| TDR | 63,948,772 | 63,948,791 | ACCGGAAGGCTCTGGAACAG |
PCR Primers
| Primer | Sequence |
| SU | AGGCTCGGCCCCTCCCTC |
| SD | TGTAACTTGGAGATCCATAGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 344 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 328 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10649A-A1 | VGB6 | C57BL/6NTac | |
| 10649A-D4 | VGB6 | C57BL/6NTac | |
| 10649A-E4 | VGB6 | C57BL/6NTac | |
| 10649A-H4 | VGB6 | C57BL/6NTac | |
| 10649A-H11 | VGB6 | C57BL/6NTac | |
| 10649A-G12 | VGB6 | C57BL/6NTac | |
