| Gene | Dlx4 |
| Chromosome | 11 |
| Deletion Start | 95,145,478 |
| Deletion End | 95,141,210 |
| Deletion Size | 4,269 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,145,230 | 95,145,249 | CGCCATCTCAGCCCTTTCAC |
| TUP | 95,145,207 | 95,145,225 | CGGCTGAACACCCGCAGGA |
| TUR | 95,145,183 | 95,145,203 | CGGTCACACTTACCTGCTTCG |
| TDF | 95,141,799 | 95,141,818 | GCAGCACAACTCGGACTCAC |
| TDP | 95,141,774 | 95,141,797 | CAAACCCAGGTAGGGCCAGTCCTG |
| TDR | 95,141,735 | 95,141,755 | CAGAGGGCAGAGAATGGGAAG |
PCR Primers
| Primer | Sequence |
| SU | CAGAGGAAAAGATGGGGAAG |
| SD | CTGCAGTCATTTGAAGGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 352 bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | 323 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10653A-A8 | VGB6 | C57BL/6NTac | |
| 10653A-C3 | VGB6 | C57BL/6NTac | |
| 10653A-E3 | VGB6 | C57BL/6NTac | |
| 10653A-H4 | VGB6 | C57BL/6NTac | |
| 10653A-G8 | VGB6 | C57BL/6NTac | |
