| Gene | Rasl10a |
| Chromosome | 11 |
| Deletion Start | 5,058,292 |
| Deletion End | 5,060,077 |
| Deletion Size | 1,786 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,058,466 | 5,058,486 | ACCTGAGTATCCGCGATGGTG |
| TUP | 5,058,487 | 5,058,508 | ACGTCGCTGGACCCGGCTCAAG |
| TUR | 5,058,517 | 5,058,536 | TCGAACCGCTACCTCAAGAC |
| TDF | 5,059,195 | 5,059,215 | TGCACCAGGACAGTCAGTTAC |
| TDP | 5,059,226 | 5,059,245 | CCGGTGGACGGCAGATCCCA |
| TDR | 5,059,265 | 5,059,285 | GCCACCCGTCAACCTACTATG |
PCR Primers
| Primer | Sequence |
| SU | GAAGGGGACGGAGCTGAAG |
| SD | CAGCCGTGGAGAGGTAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 336 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | 316 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10655A-B12 | VGB6 | C57BL/6NTac | |
| 10655A-G10 | VGB6 | C57BL/6NTac | |
