| Gene | Gpr50 |
| Chromosome | X |
| Deletion Start | 71,663,777 |
| Deletion End | 71,668,090 |
| Deletion Size | 4,314 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,663,913 | 71,663,932 | GCGCAATGGTCATCACAGTC |
| TUP | 71,663,934 | 71,663,960 | TCGTAGACCTGATCGGGAACTCCATGG |
| TUR | 71,663,974 | 71,663,995 | TCGGAGCTTCTTGTTCTTGGTC |
| TDF | 71,667,938 | 71,667,958 | CTGCCTCTGTCCTTCCTTCTG |
| TDP | 71,667,959 | 71,667,981 | TACCCTTCGAGCTTGCTGCCACC |
| TDR | 71,668,016 | 71,668,037 | AGCACGACCTTGCGGTAATCAC |
PCR Primers
| Primer | Sequence |
| SU | GTAGTCGGAAGCAAGCTGAC |
| SD | GACAGCGAGGCTTAGGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 412 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | 325 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10656C-E1 | VGB6 | C57BL/6NTac | |
| 10656D-A4 | VGB6 | C57BL/6NTac | |
| 10656D-H2 | VGB6 | C57BL/6NTac | |
