| Gene | Serpinb1c |
| Chromosome | 13 |
| Deletion Start | 32,897,095 |
| Deletion End | 32,881,814 |
| Deletion Size | 15,282 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,896,323 | 32,896,343 | ACAGGACCCACATCCTCTCAG |
| TUP | 32,896,299 | 32,896,322 | CCCACCTCTGGGAGACAGCAGGTC |
| TUR | 32,896,258 | 32,896,278 | TTCACACCCATGAAGGAAGAC |
| TDF | 32,881,943 | 32,881,962 | CATGCCAGGCACTGTTGTTG |
| TDP | 32,881,916 | 32,881,940 | TGCTGCCTGATGCCCATGGAATTCA |
| TDR | 32,881,863 | 32,881,881 | CACATGAGCCGTGGGATTG |
PCR Primers
| Primer | Sequence |
| SU | ACCCTAGGTCCTGGTCTTG |
| SD | TACAGGTTACCTTATGGTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10657A-B5 | VGB6 | C57BL/6NTac | |
| 10657A-F11 | VGB6 | C57BL/6NTac | |
