| Gene | Fndc4 |
| Chromosome | 5 |
| Deletion Start | 31,295,330 |
| Deletion End | 31,293,358 |
| Deletion Size | 1,973 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,295,072 | 31,295,091 | TCACTCATCTCAGAGCCAAC |
| TUP | 31,295,050 | 31,295,071 | TCAGCCACTGTGTCCTGGGACG |
| TUR | 31,294,997 | 31,295,021 | GATTCATACTTGCTGGGAAATGGAG |
| TDF | 31,294,290 | 31,294,308 | CAGGCTCGGTGCAATGATG |
| TDP | 31,294,264 | 31,294,288 | ACCCAGCCTGACTTCAACCTCCAGC |
| TDR | 31,294,202 | 31,294,225 | ACAGCCATTCTCTGGGTTACAAAG |
PCR Primers
| Primer | Sequence |
| SU | TGACCGACCACGAGGACCC |
| SD | TGATATCCACTCCCAAGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 340 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | 231 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10658A-A10 | VGB6 | C57BL/6NTac | |
| 10658A-A12 | VGB6 | C57BL/6NTac | |
| 10658A-E1 | VGB6 | C57BL/6NTac | |
| 10658A-E10 | VGB6 | C57BL/6NTac | |
| 10658A-F4 | VGB6 | C57BL/6NTac | |
| 10658A-F5 | VGB6 | C57BL/6NTac | |
