| Gene | Dkk4 |
| Chromosome | 8 |
| Deletion Start | 22,624,167 |
| Deletion End | 22,627,368 |
| Deletion Size | 3,202 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,624,701 | 22,624,725 | ACAGCAGATGTGTAGGAGTTAGGAC |
| TUP | 22,624,727 | 22,624,753 | TGAACACATTAGGACATGACCCAGCAC |
| TUR | 22,624,761 | 22,624,780 | ACAATTCCCGTGCCGTAGAC |
| TDF | 22,626,784 | 22,626,803 | GCAGGACAGGAGGGAGAAAG |
| TDP | 22,626,805 | 22,626,831 | TGTCTTAGAACCTCTGACTGTGGCCCT |
| TDR | 22,626,833 | 22,626,851 | TGGCGAGCACAGCAAAGTC |
PCR Primers
| Primer | Sequence |
| SU | AGAAGAGGGGCCACGTCTG |
| SD | TGCTAGGCAGTCCACTGTGT |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 328 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 312 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10660A-A7 | VGB6 | C57BL/6NTac | |
| 10660A-B4 | VGB6 | C57BL/6NTac | |
| 10660A-F11 | VGB6 | C57BL/6NTac | |
| 10660A-F4 | VGB6 | C57BL/6NTac | |
