| Gene | Emilin3 |
| Chromosome | 2 |
| Deletion Start | 160,912,168 |
| Deletion End | 160,907,529 |
| Deletion Size | 4,640 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 160,911,332 | 160,911,356 | CAGGGCAGATGTCCTATAAGAGAAG |
| TUP | 160,911,308 | 160,911,331 | ACACGAGTACTGCTCTCGGGAGCA |
| TUR | 160,911,287 | 160,911,305 | GCCTCGGCTTTGCCATTTC |
| TDF | 160,907,793 | 160,907,813 | CCCAGGAACTCCAGCATACAG |
| TDP | 160,907,763 | 160,907,787 | CACTTTGACCAGAGGGTGGCCCAAG |
| TDR | 160,907,731 | 160,907,750 | CAACAGGCCCAACCTCTCAC |
PCR Primers
| Primer | Sequence |
| SU | GCGTCCCGCCTGCTCGTTTC |
| SD | GCAGCGTGCCATCTCAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 457 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 327 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10664A-A5 | VGB6 | C57BL/6NTac | |
| 10664A-C11 | VGB6 | C57BL/6NTac | |
| 10664A-G2 | VGB6 | C57BL/6NTac | |
| 10664A-H1 | VGB6 | C57BL/6NTac | |
