| Gene | Ush1g |
| Chromosome | 11 |
| Deletion Start | 115,321,853 |
| Deletion End | 115,316,916 |
| Deletion Size | 4,938 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,321,803 | 115,321,824 | TGGCTATCTGGAACTCCTCAAG |
| TUP | 115,321,779 | 115,321,801 | AGGCCACCCGGAAGGAGCTGAAT |
| TUR | 115,321,755 | 115,321,774 | GGTCATGCCATCCTCGTCAG |
| TDF | 115,317,206 | 115,317,227 | CCCAATAGGTCCAACAGCAATC |
| TDP | 115,317,183 | 115,317,205 | TTGGAGGCACAACTGTGGCTGTA |
| TDR | 115,317,143 | 115,317,164 | GGCAGGAGTAGGGAATGGTTTG |
PCR Primers
| Primer | Sequence |
| SU | CTGGGCAGTGAGAGATTTCC |
| SD | GCCATCAGAAGTGCTGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 380 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 318 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10667A-B11 | VGB6 | C57BL/6NTac | |
| 10667B-D5 | VGB6 | C57BL/6NTac | |
| 10667B-E12 | VGB6 | C57BL/6NTac | |
| 10667B-A12 | VGB6 | C57BL/6NTac | |
| 10667C-C2 | VGB6 | C57BL/6NTac | |
| 10667A-A6 | VGB6 | C57BL/6NTac | |
