| Gene | Amigo1 |
| Chromosome | 3 |
| Deletion Start | 108,187,190 |
| Deletion End | 108,188,665 |
| Deletion Size | 1,476 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,187,513 | 108,187,534 | ACCCAACCTTAGGTACTTGGAC |
| TUP | 108,187,538 | 108,187,562 | TCCTCCAACCATCTTCACACGCTGG |
| TUR | 108,187,565 | 108,187,585 | CAGGTCGCTGAACAGGAACTC |
| TDF | 108,187,994 | 108,188,015 | TGCAGCGAGTACAAGGAAAGTG |
| TDP | 108,188,022 | 108,188,045 | AGGCTCACCTGGGAGACACCTTGA |
| TDR | 108,188,062 | 108,188,082 | TTGGTCATGCCTTGCTGTTTG |
PCR Primers
| Primer | Sequence |
| SU | GGCCTTTGCGCCTATGCCTC |
| SD | GAGAATAGCCAAGGCTTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 415 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 333 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10669A-H9 | VGB6 | C57BL/6NTac | |
| 10669A-F8 | VGB6 | C57BL/6NTac | |
| 10669A-E11 | VGB6 | C57BL/6NTac | |
| 10669A-E9 | VGB6 | C57BL/6NTac | |
