| Gene | Trim38 |
| Chromosome | 13 |
| Deletion Start | 23,782,553 |
| Deletion End | 23,791,498 |
| Deletion Size | 8,946 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,783,458 | 23,783,481 | TCATGCCATAGGAACTTCCTCATC |
| TUP | 23,783,484 | 23,783,505 | TGCCAAGGCAAGCAGCACTGTA |
| TUR | 23,783,517 | 23,783,539 | GAGTCTGGGTTCAGCTATGTTTC |
| TDF | 23,791,172 | 23,791,195 | TTGGGAGTCTGTGTAGAGAATGTG |
| TDP | 23,791,205 | 23,791,234 | TTTAACATGAAGAAGGAGCCTGAGTCTGGA |
| TDR | 23,791,254 | 23,791,273 | TTCAAGGCCGTCCTCTTCAG |
PCR Primers
| Primer | Sequence |
| SU | AATTTTTATTAGGTTGAATG |
| SD | AGATAAAAGTGGTCTTTCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 351 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | 360 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10670A-A1 | VGB6 | C57BL/6NTac | |
| 10670B-E6 | VGB6 | C57BL/6NTac | |
