| Gene | Rnf215 |
| Chromosome | 11 |
| Deletion Start | 4,135,256 |
| Deletion End | 4,140,509 |
| Deletion Size | 5,254 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,135,588 | 4,135,607 | GCCATGACCCTCGGATAGAC |
| TUP | 4,135,608 | 4,135,633 | CCCGCTGTCTTCATCCAGAAGCTAGC |
| TUR | 4,135,655 | 4,135,675 | CTCCTGAAGGCTGGGAAGTAG |
| TDF | 4,139,750 | 4,139,771 | GCAGCTATGGAACGCCATCTTG |
| TDP | 4,139,773 | 4,139,795 | TTGTGGCCATGCTCCTGTGTACA |
| TDR | 4,139,827 | 4,139,847 | GCTCCTGCTGGTTCTGTCTTG |
PCR Primers
| Primer | Sequence |
| SU | AACCGCACCTATCTCCACTG |
| SD | GGTGTAATGGCCGTATGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 405 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | 358 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10671A-A3 | VGB6 | C57BL/6NTac | |
| 10671A-A12 | VGB6 | C57BL/6NTac | |
| 10671A-E5 | VGB6 | C57BL/6NTac | |
| 10671A-E7 | VGB6 | C57BL/6NTac | |
