| Gene | C030019I05Rik |
| Chromosome | 11 |
| Deletion Start | 46,237,659 |
| Deletion End | 46,238,372 |
| Deletion Size | 714 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,237,777 | 46,237,799 | CCGGATACCTGCGCTATAACTTC |
| TUP | 46,237,800 | 46,237,823 | CATCACGAGGAGAAGGTGCCTCGA |
| TUR | 46,237,826 | 46,237,846 | TCCAATGCCACAGAGGTGATG |
| TDF | 46,238,078 | 46,238,098 | CGAACCTCGGTGGTCTTACAG |
| TDP | 46,238,101 | 46,238,121 | CCGGCCAAATGGAGGAAGCCA |
| TDR | 46,238,130 | 46,238,149 | GTCTCCTCGGGCCATCTTAC |
PCR Primers
| Primer | Sequence |
| SU | CATCTTGAGCCTGAGGGCTG |
| SD | GTGCCAGATTCAGGTCTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 368 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 373 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10672A-A1 | VGB6 | C57BL/6NTac | |
| 10672A-B7 | VGB6 | C57BL/6NTac | |
| 10672A-F6 | VGB6 | C57BL/6NTac | |
| 10672A-G3 | VGB6 | C57BL/6NTac | |
| 10672A-H3 | VGB6 | C57BL/6NTac | |
| 10672A-E1 | VGB6 | C57BL/6NTac | |
