| Gene | Gpr183 |
| Chromosome | 14 |
| Deletion Start | 121,955,104 |
| Deletion End | 121,954,015 |
| Deletion Size | 1,090 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,955,037 | 121,955,057 | TGATCTCTATGCCCACCACAG |
| TUP | 121,955,010 | 121,955,036 | CACAGCCAGGGTATTAATGCCTCTGCA |
| TUR | 121,954,978 | 121,954,997 | CCACCAGCCCAATGATGAAG |
| TDF | 121,954,514 | 121,954,533 | TTCTGCTCGGAGCCTGTCTG |
| TDP | 121,954,490 | 121,954,513 | CTGGGCTACGTGCTGCCTATCACA |
| TDR | 121,954,432 | 121,954,451 | TCTGCTTGGCAGTCCTGAAG |
PCR Primers
| Primer | Sequence |
| SU | GGTTTCTCTCTGAAGCTAGTG |
| SD | TACACTGGCAATATACAGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 379 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | 369 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10674A-B5 | VGB6 | C57BL/6NTac | |
| 10674A-B9 | VGB6 | C57BL/6NTac | |
| 10674A-D6 | VGB6 | C57BL/6NTac | |
