| Gene | Taar5 |
| Chromosome | 10 |
| Deletion Start | 23,970,709 |
| Deletion End | 23,971,746 |
| Deletion Size | 1,038 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,970,996 | 23,971,015 | GAGCTGCTGGTTCTTTGGAG |
| TUP | 23,971,016 | 23,971,042 | ACTTCCTGTGCCGTCTGCATACCTATC |
| TUR | 23,971,060 | 23,971,082 | CAGAGATGGAAGATGGAGGTGAG |
| TDF | 23,971,603 | 23,971,626 | TGTAATCCCATCATCTACGTCTTC |
| TDP | 23,971,629 | 23,971,654 | CTACCGGTGGTTTAGGAAGGCACTGA |
| TDR | 23,971,680 | 23,971,699 | CAGTCGGTGTCCTTGGTGAG |
PCR Primers
| Primer | Sequence |
| SU | GAAGAGCCTTGTGTGATAGAC |
| SD | GACTTCCTCCTGTGCTTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 405 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | 348 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10675A-A8 | VGB6 | C57BL/6NTac | |
| 10675A-A9 | VGB6 | C57BL/6NTac | |
| 10675A-A10 | VGB6 | C57BL/6NTac | |
| 10675A-C4 | VGB6 | C57BL/6NTac | |
| 10675A-C8 | VGB6 | C57BL/6NTac | |
| 10675A-F11 | VGB6 | C57BL/6NTac | |
