| Gene | Serpinb1a |
| Chromosome | 13 |
| Deletion Start | 32,850,403 |
| Deletion End | 32,842,696 |
| Deletion Size | 7,708 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,849,589 | 32,849,612 | TGCTCTTGGTGTTCCTTAATTCTG |
| TUP | 32,849,550 | 32,849,578 | TCTGCCACCTTTGGAAATCATGACAGGAG |
| TUR | 32,849,518 | 32,849,535 | GCAGCAGGTGCAGTTAGG |
| TDF | 32,842,855 | 32,842,872 | TCGGCACAATCCCACATC |
| TDP | 32,842,827 | 32,842,853 | AATGTGCTCTTCCTTGGCAGGGTTTGT |
| TDR | 32,842,790 | 32,842,812 | TGCCTTGTATCTGTAAAGTCTCC |
PCR Primers
| Primer | Sequence |
| SU | CTTCCCTGTCACCCACATC |
| SD | ATCCACGCTACCCCAGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10677A-F10 | VGB6 | C57BL/6NTac | |
| 10677A-C3 | VGB6 | C57BL/6NTac | |
| 10677A-H12 | VGB6 | C57BL/6NTac | |
