| Gene | Zmiz2 |
| Chromosome | 11 |
| Deletion Start | 6,395,614 |
| Deletion End | 6,405,115 |
| Deletion Size | 9,502 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,396,454 | 6,396,471 | CCATCAGCCTGTCAGTCC |
| TUP | 6,396,474 | 6,396,500 | CAGAAGGGTTGGATGTTGCCTGCTCCT |
| TUR | 6,396,541 | 6,396,562 | CCTCCACTCACTTCTTCCATTC |
| TDF | 6,404,884 | 6,404,904 | GGCACTTTAGCATTCCCACTG |
| TDP | 6,404,914 | 6,404,938 | CCAGAACAGCTTGGCCACTGGAAGT |
| TDR | 6,404,971 | 6,404,990 | GCTGAGGGCAAAGCAAACAC |
PCR Primers
| Primer | Sequence |
| SU | CTGAGGGTTCCTGGCTTCC |
| SD | AACCATTCTGGAGCAGTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 404 bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | 302 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10680A-C9 | VGB6 | C57BL/6NTac | |
| 10680B-E8 | VGB6 | C57BL/6NTac | |
| 10680C-D1 | VGB6 | C57BL/6NTac | |
| 10680D-E4 | VGB6 | C57BL/6NTac | |
