| Gene | Hes2 |
| Chromosome | 4 |
| Deletion Start | 152,159,654 |
| Deletion End | 152,160,581 |
| Deletion Size | 928 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 152,159,753 | 152,159,774 | GCGATGGCGGTCTTTCTAACTG |
| TUP | 152,159,775 | 152,159,794 | TGGTCTCAGCGCCTTCTCGC |
| TUR | 152,159,804 | 152,159,822 | CAGCAGCGGCTTTAGGTTC |
| TDF | 152,160,262 | 152,160,283 | CTCCAGTTTGCACCGTACTCAG |
| TDP | 152,160,285 | 152,160,309 | ACCTGAGCCTCACATCTGCTCCCTG |
| TDR | 152,160,341 | 152,160,359 | GCCAGACAGGCTCGGTAAC |
PCR Primers
| Primer | Sequence |
| SU | CGCTGGCCCGACTCAATGG |
| SD | CACCACTTCTTCCAGTCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 328 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | 317 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10681B-C11 | VGB6 | C57BL/6NTac | |
| 10681C-H2 | VGB6 | C57BL/6NTac | |
