| Gene | Tspan7 |
| Chromosome | X |
| Deletion Start | 10,064,994 |
| Deletion End | 10,065,070 |
| Deletion Size | 77 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,485,164 | 10,485,185 | TCGAGGAGAATGGAGACCAAAC |
| TUP | 10,485,187 | 10,485,215 | TGTGATAACCTGTCTCAAAACCCTCCTCA |
| TUR | 10,485,216 | 10,485,238 | CCAGAAGACGAAGGAGTAGATGA |
| TDF | 10,485,169 | 10,485,191 | GAGAATGGAGACCAAACCTGTGA |
| TDP | | | AACCTGTCTCAAAACCC |
| TDR | 10,485,210 | 10,485,232 | GACGAAGGAGTAGATGATGAGGA |
PCR Primers
| Primer | Sequence |
| SU | CCAGCCTCTAGGCTCTCAG |
| SD | CTTTCCCCAGGGGCTGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 64 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10682A-G12 | VGB6 | C57BL/6NTac | |
| 10682B-C11 | VGB6 | C57BL/6NTac | |
| 10682B-F1 | VGB6 | C57BL/6NTac | |
| 10682B-F8 | VGB6 | C57BL/6NTac | |
