| Gene | Serpinb9b |
| Chromosome | 13 |
| Deletion Start | 33,029,446 |
| Deletion End | 33,040,073 |
| Deletion Size | 10,628 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,029,486 | 33,029,506 | GGCACCTTTGCCATCCATCTA |
| TUP | 33,029,508 | 33,029,537 | TGAAGATGTTATGTCAAAGCAACCCTTCCA |
| TUR | 33,029,591 | 33,029,610 | GTCTGCTCCTTTGCACCCAG |
| TDF | 33,039,980 | 33,040,000 | GTGAGTTCACACTCCAGCATC |
| TDP | 33,040,002 | 33,040,024 | CCACCACTCCTACAGCTCCGTGA |
| TDR | 33,040,025 | 33,040,043 | TCCCTCTGTTTGCCCATTC |
PCR Primers
| Primer | Sequence |
| SU | CAGAGTTGCTGAAGGCAGG |
| SD | CTTGTACGGTGAAATCACTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 549 bp | - | + | + |
| TUF | TUR | 125 bp | + | + | - |
| TDF | TDR | 64 bp | + | + | - |
| NeoFwd | SD | 252 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10690C-B4 | VGB6 | C57BL/6NTac | |
| 10690D-F7 | VGB6 | C57BL/6NTac | |
