| Gene | P2ry6 |
| Chromosome | 7 |
| Deletion Start | 100,939,147 |
| Deletion End | 100,938,140 |
| Deletion Size | 1,008 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,939,112 | 100,939,130 | CATCCAGGCTCCAGGCTTG |
| TUP | 100,939,085 | 100,939,107 | CCACCACCTGCGTCTACCGTGAG |
| TUR | 100,939,004 | 100,939,024 | GGCAATGACGCAGATGTTCAG |
| TDF | 100,938,378 | 100,938,400 | AGCTTCCTGCCTTTCCACATCAC |
| TDP | 100,938,349 | 100,938,371 | AGCCTACTTGGCTGTGCGCTCCA |
| TDR | 100,938,323 | 100,938,342 | CAGCACAGGGCAAGAGACAC |
PCR Primers
| Primer | Sequence |
| SU | ACACAGAGAAACCCTGTGTC |
| SD | TGGGGTTTCACTGTGTAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 127 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10694B-D5 | VGB6 | C57BL/6NTac | |
| 10694C-G7 | VGB6 | C57BL/6NTac | |
