| Gene | Oxgr1 |
| Chromosome | 14 |
| Deletion Start | 120,022,790 |
| Deletion End | 120,021,348 |
| Deletion Size | 1,443 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,022,707 | 120,022,724 | TGCACCGACGAGCAAATC |
| TUP | 120,022,679 | 120,022,706 | TCATTCAAGATGCAGTACCTTCCCGTCA |
| TUR | 120,022,649 | 120,022,668 | GGAAGCCCACGAGGAAGATG |
| TDF | 120,021,909 | 120,021,929 | CCATTAGCTGCTCTCAACACC |
| TDP | 120,021,877 | 120,021,906 | TGGCAACCTGCTGTTATATGTTGTGGTCAG |
| TDR | 120,021,826 | 120,021,845 | CCACTGGCTTTGCATCTCAC |
PCR Primers
| Primer | Sequence |
| SU | CTAGTACCTTCATGGGACTC |
| SD | TGAGCCTTCCCATCTTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 351 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | 392 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10698A-C8 | VGB6 | C57BL/6NTac | |
| 10698B-F2 | VGB6 | C57BL/6NTac | |
| 10698C-B4 | VGB6 | C57BL/6NTac | |
| 10698C-C2 | VGB6 | C57BL/6NTac | |
| 10698C-D12 | VGB6 | C57BL/6NTac | |
| 10698C-E2 | VGB6 | C57BL/6NTac | |
| 10698D-A8 | VGB6 | C57BL/6NTac | |
