| Gene | Sebox |
| Chromosome | 11 |
| Deletion Start | 78,503,535 |
| Deletion End | 78,504,411 |
| Deletion Size | 877 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,503,784 | 78,503,801 | TCAGTGTCGGGCAGTTGG |
| TUP | 78,503,811 | 78,503,835 | AGCGGGTATTTGCAGCTAGGCCCTA |
| TUR | 78,503,840 | 78,503,858 | GCTCACGGGTGCTGATGTC |
| TDF | 78,504,106 | 78,504,128 | CTCCTGTTCTCTTCCAGACACTC |
| TDP | 78,504,130 | 78,504,151 | CCAGCTACCTTGGGACCCTGGA |
| TDR | 78,504,153 | 78,504,172 | GTGCAGAGGATGGCTTGATG |
PCR Primers
| Primer | Sequence |
| SU | TCTTGGTGCACACCAATCTG |
| SD | CCCAAAAGGACCAGGATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 330 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | 287 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10699C-B7 | VGB6 | C57BL/6NTac | |
| 10699C-F4 | VGB6 | C57BL/6NTac | |
| 10699C-E11 | VGB6 | C57BL/6NTac | |
| 10699C-E10 | VGB6 | C57BL/6NTac | |
| 10699C-E5 | VGB6 | C57BL/6NTac | |
| 10699C-C12 | VGB6 | C57BL/6NTac | |
