| Gene | Pink1 |
| Chromosome | 4 |
| Deletion Start | 138,326,216 |
| Deletion End | 138,313,899 |
| Deletion Size | 12,318 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,325,706 | 138,325,728 | GGGTTAGGAGAGTGAGTGATCTG |
| TUP | 138,325,669 | 138,325,693 | CCAAATTGGACAGCAAGGGCGGAGT |
| TUR | 138,325,622 | 138,325,644 | CCCTACACCACTAGGATTCTGAG |
| TDF | 138,314,405 | 138,314,429 | GTGCTAGAGATCAGAGCAGTGTATG |
| TDP | 138,314,375 | 138,314,399 | CCTTCATGACAGAGGTGTCCCAGCA |
| TDR | 138,314,351 | 138,314,370 | CCAGGACCTTGCCCATGTTG |
PCR Primers
| Primer | Sequence |
| SU | ACCAGTGAAGTTGTTCACAAC |
| SD | CTACTTCATTCCCTGTCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 329 bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 341 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10701A-A5 | VGB6 | C57BL/6NTac | |
| 10701A-A10 | VGB6 | C57BL/6NTac | |
| 10701A-G10 | VGB6 | C57BL/6NTac | |
| 10701A-D3 | VGB6 | C57BL/6NTac | |
