| Gene | Omg |
| Chromosome | 11 |
| Deletion Start | 79,503,018 |
| Deletion End | 79,501,679 |
| Deletion Size | 1,340 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,502,884 | 79,502,905 | GGACTGTTCAGGCAGAAACTTG |
| TUP | 79,502,856 | 79,502,880 | ACATTACCACCTGGACTGCAGGAGA |
| TUR | 79,502,760 | 79,502,784 | TGTTTGAAATATCCAGGGTTCTCAG |
| TDF | 79,501,819 | 79,501,839 | CGACAAAGCACAACCCTCAAC |
| TDP | 79,501,790 | 79,501,816 | ACGGAGGGAAGAAACCACTGCAAATGG |
| TDR | 79,501,756 | 79,501,775 | CCAAGCACTAGCCGCAGAAG |
PCR Primers
| Primer | Sequence |
| SU | TGAAGGATTAGGCATTTGGAC |
| SD | AGACAGGAAAATAGCAGCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 304 bp | - | + | + |
| TUF | TUR | 146 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | 285 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10706A-D9 | VGB6 | C57BL/6NTac | |
| 10706A-C9 | VGB6 | C57BL/6NTac | |
| 10706A-B3 | VGB6 | C57BL/6NTac | |
