| Gene | Gpr18 |
| Chromosome | 14 |
| Deletion Start | 121,912,608 |
| Deletion End | 121,911,596 |
| Deletion Size | 1,013 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,912,285 | 121,912,303 | TTACCCAAGCCTCGCTCTG |
| TUP | 121,912,257 | 121,912,284 | TGGCTTCTTGCTTTCATTAGTGCTGACA |
| TUR | 121,912,199 | 121,912,218 | GCCTTGCCGGTGTTCTTCAG |
| TDF | 121,911,923 | 121,911,943 | GCCCAAGGTCAAGGAGAAGTC |
| TDP | 121,911,894 | 121,911,920 | TACGGATCATCATGACCCTCCTGCTGC |
| TDR | 121,911,851 | 121,911,871 | ACGGCAAAGCAGATGTGGAAG |
PCR Primers
| Primer | Sequence |
| SU | CGAGTAGTCAGTTAGAGAGG |
| SD | TGTTTGTTTGGATCACAGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 328 bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | 349 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10707C-A3 | VGB6 | C57BL/6NTac | |
| 10707C-B9 | VGB6 | C57BL/6NTac | |
| 10707C-G6 | VGB6 | C57BL/6NTac | |
| 10707C-F12 | VGB6 | C57BL/6NTac | |
| 10707D-A3 | VGB6 | C57BL/6NTac | |
| 10707D-C2 | VGB6 | C57BL/6NTac | |
| 10707D-C6 | VGB6 | C57BL/6NTac | |
