| Gene | Fgfrl1 |
| Chromosome | 5 |
| Deletion Start | 108,695,055 |
| Deletion End | 108,706,348 |
| Deletion Size | 11,294 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,695,781 | 108,695,800 | TGTTGCAGGATACACCACAC |
| TUP | 108,695,803 | 108,695,830 | TGGCTTGGCTACATCACTCGTCTTCTGG |
| TUR | 108,695,858 | 108,695,878 | ATCAGCTCTGGGTGCCCTCTG |
| TDF | 108,706,196 | 108,706,215 | TCATGTGGAGGGCAAGGTTC |
| TDP | 108,706,218 | 108,706,244 | CAACACCAGCATGTCCACTATCAGTGC |
| TDR | 108,706,259 | 108,706,278 | TCAGGACACAGTGCTTGGAG |
PCR Primers
| Primer | Sequence |
| SU | GGAATCCAGACGGTGTTTGG |
| SD | CCATGACCACTAAGACATGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 376 bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 319 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10708B-G5 | VGB6 | C57BL/6NTac | |
| 10708E-C4 | VGB6 | C57BL/6NTac | |
| 10708E-G4 | VGB6 | C57BL/6NTac | |
| 10708E-E9 | VGB6 | C57BL/6NTac | |
