| Gene | Snph |
| Chromosome | 2 |
| Deletion Start | 151,601,024 |
| Deletion End | 151,593,380 |
| Deletion Size | 7,645 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 151,600,780 | 151,600,803 | CAAATGACACGGTGGAGATAACAG |
| TUP | 151,600,752 | 151,600,777 | ATCTGAAGGTTCCCATCAGCACCAGC |
| TUR | 151,600,707 | 151,600,726 | GGGCCAAAGGGAGAAACTAC |
| TDF | 151,593,953 | 151,593,973 | GGGCCATCCAGACAGACTTTG |
| TDP | 151,593,929 | 151,593,952 | TGCAGTACCAGCCTGACCTGAACA |
| TDR | 151,593,881 | 151,593,899 | TGAGGACTGAACCGCACAC |
PCR Primers
| Primer | Sequence |
| SU | TGAATTCACTGGTGGAGGCA |
| SD | CAGATCAATACAGCCAGAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 341 bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | 373 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10709A-D3 | VGB6 | C57BL/6NTac | |
| 10709A-D4 | VGB6 | C57BL/6NTac | |
| 10709A-E6 | VGB6 | C57BL/6NTac | |
