| Gene | Zpbp2 |
| Chromosome | 11 |
| Deletion Start | 98,551,277 |
| Deletion End | 98,558,356 |
| Deletion Size | 7,080 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,551,496 | 98,551,516 | GTAGGGCACCCAGGTAATACG |
| TUP | 98,551,517 | 98,551,540 | TGCCTCTTCTCACGCAAGGCTTGT |
| TUR | 98,551,564 | 98,551,584 | TCCCTGCCTGAAGAACCTAAG |
| TDF | 98,558,185 | 98,558,202 | CTGACAGCCTGCGTTTCC |
| TDP | 98,558,204 | 98,558,229 | TTGCAGTGGTCTGTAGTCCTGGAACG |
| TDR | 98,558,239 | 98,558,260 | TCTGACAGGTGACATCCACATC |
PCR Primers
| Primer | Sequence |
| SU | CCATCTGCGAGCGCTTG |
| SD | TTCAGTTTGTAGAAAACAGAACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 340 bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 328 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10710A-G11 | VGB6 | C57BL/6NTac | |
| 10710C-F12 | VGB6 | C57BL/6NTac | |
| 10710D-G7 | VGB6 | C57BL/6NTac | |
| 10710D-E12 | VGB6 | C57BL/6NTac | |
| 10710D-D12 | VGB6 | C57BL/6NTac | |
