| Gene | Olfr267 |
| Chromosome | 4 |
| Deletion Start | 58,785,717 |
| Deletion End | 58,784,757 |
| Deletion Size | 961 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,785,636 | 58,785,660 | CCGAGGTTAGAGATTGTTCTGTTTG |
| TUP | 58,785,603 | 58,785,632 | TCAGCCTTGTCATGTATCTGGTTACCATCC |
| TUR | 58,785,557 | 58,785,577 | AGGCGTGAATCCAGGACAGTG |
| TDF | 58,785,111 | 58,785,133 | GGTTATGCTGGTGGTCAGTATTC |
| TDP | 58,785,084 | 58,785,110 | TCCTTCTGCCCATTCCAATGCTCTTGA |
| TDR | 58,785,023 | 58,785,046 | CTTCCCTCTGTTGAACTGATCCTC |
PCR Primers
| Primer | Sequence |
| SU | GGTTAAACAAATGGATTGAG |
| SD | ACTGACTACATTTATCCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 347 bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 111 bp | + | + | - |
| NeoFwd | SD | 375 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10711A-C1 | VGB6 | C57BL/6NTac | |
| 10711B-H5 | VGB6 | C57BL/6NTac | |
| 10711C-G7 | VGB6 | C57BL/6NTac | |
| 10711C-E7 | VGB6 | C57BL/6NTac | |
| 10711C-D3 | VGB6 | C57BL/6NTac | |
| 10711C-D9 | VGB6 | C57BL/6NTac | |
| 10711C-D4 | VGB6 | C57BL/6NTac | |
| 10711D-A8 | VGB6 | C57BL/6NTac | |
