| Gene | Ifnk |
| Chromosome | 4 |
| Deletion Start | 35,152,056 |
| Deletion End | 35,153,818 |
| Deletion Size | 1,763 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,152,428 | 35,152,449 | TGCTTCATGGACGAGGAGAAAG |
| TUP | 35,152,454 | 35,152,478 | CAGGGAACATCCTCACTCCGAGGAC |
| TUR | 35,152,488 | 35,152,506 | TGCCCAACTCCAGGTAGAC |
| TDF | 35,153,410 | 35,153,434 | TCAGTGGTAGCTGTACTTGTCTAAC |
| TDP | 35,153,436 | 35,153,463 | TGCATAAGGCACTTGGACTGGATTCTCA |
| TDR | 35,153,472 | 35,153,491 | ATGCCTTCCCCTAACCTCAC |
PCR Primers
| Primer | Sequence |
| SU | TTGACAAACTAAGCCACACC |
| SD | TAAACGCCATAATGTATAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 356 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 294 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10712A-A11 | VGB6 | C57BL/6NTac | |
| 10712A-B8 | VGB6 | C57BL/6NTac | |
| 10712A-C4 | VGB6 | C57BL/6NTac | |
| 10712A-D10 | VGB6 | C57BL/6NTac | |
| 10712A-F3 | VGB6 | C57BL/6NTac | |
| 10712A-C12 | VGB6 | C57BL/6NTac | |
