| Gene | Lrrn3 |
| Chromosome | 12 |
| Deletion Start | 41,454,313 |
| Deletion End | 41,452,190 |
| Deletion Size | 2,124 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,453,683 | 41,453,705 | GGACATGAACTTTCAGCCTCTTG |
| TUP | 41,453,658 | 41,453,682 | TCAAGCTTCGCAGCCTGGTTATAGC |
| TUR | 41,453,626 | 41,453,647 | CGTCGTCTGGTATTTCCGTGAG |
| TDF | 41,453,087 | 41,453,108 | AGGCCAGAATGTTCGGCAAGTG |
| TDP | 41,453,055 | 41,453,082 | TCAGGGATATGATGGAAATTTGCCTCCC |
| TDR | 41,453,021 | 41,453,043 | CAGGTCAGAAGGAAAGCTCTCAG |
PCR Primers
| Primer | Sequence |
| SU | ATTGCTCCATATCCTGTGAC |
| SD | AAATGTTCACGTTTAACTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 422 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | 364 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10716E-F9 | VGB6 | C57BL/6NTac | |
| 10716E-E1 | VGB6 | C57BL/6NTac | |
| 10716D-H8 | VGB6 | C57BL/6NTac | |
