| Gene | Sowaha |
| Chromosome | 11 |
| Deletion Start | 53,479,904 |
| Deletion End | 53,478,239 |
| Deletion Size | 1,666 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,479,517 | 53,479,536 | CCCACAGCGATCAGGAGAAC |
| TUP | 53,479,489 | 53,479,510 | AGGACTCGTCCGCACCATCCGA |
| TUR | 53,479,447 | 53,479,466 | TGGGTGACCTCTGAGGGTAG |
| TDF | 53,478,447 | 53,478,467 | GTCCTGGCCGATGACTTGATG |
| TDP | 53,478,418 | 53,478,442 | AGGACCTGGCTCGTGGCTTGAAGAA |
| TDR | 53,478,388 | 53,478,408 | GCACCCAAGAACTTGCTGAAG |
PCR Primers
| Primer | Sequence |
| SU | GGCAGCTGTCACCTGTAAGC |
| SD | ACCAGCTGCTGCCCCTTAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 377 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 344 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10717A-H8 | VGB6 | C57BL/6NTac | |
| 10717A-B1 | VGB6 | C57BL/6NTac | |
| 10717A-H2 | VGB6 | C57BL/6NTac | |
| 10717A-F12 | VGB6 | C57BL/6NTac | |
| 10717A-G1 | VGB6 | C57BL/6NTac | |
| 10717A-A2 | VGB6 | C57BL/6NTac | |
