| Gene | Gpr81 |
| Chromosome | 5 |
| Deletion Start | 123,879,599 |
| Deletion End | 123,878,552 |
| Deletion Size | 1,048 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 123,879,542 | 123,879,563 | ATCTCCCAAGTGATGCCTCCTC |
| TUP | 123,879,516 | 123,879,540 | ACTCATCCTGGTCTTCGTGCTTGGC |
| TUR | 123,879,483 | 123,879,501 | GAAGCCGCACAGGGCTATG |
| TDF | 123,878,664 | 123,878,684 | ACGCGGAGGTCAGAAGAGATG |
| TDP | 123,878,633 | 123,878,658 | TTCGAACCTCTGCAGTAAGAGCTCCA |
| TDR | 123,878,602 | 123,878,623 | GATGGCCTCTGGGAACGATTTG |
PCR Primers
| Primer | Sequence |
| SU | TCGTGGGAAACCGCTTGCAC |
| SD | ACCCACGGTTTCCTTCACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 380 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 323 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10719B-D4 | VGB6 | C57BL/6NTac | |
