| Gene | Olfr272 |
| Chromosome | 4 |
| Deletion Start | 52,911,789 |
| Deletion End | 52,910,814 |
| Deletion Size | 976 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,911,421 | 52,911,442 | GATGGCACTTGACCGCTTTATG |
| TUP | 52,911,390 | 52,911,419 | CCATCTGTTACCCACTGAGGTATCCTGTCA |
| TUR | 52,911,338 | 52,911,356 | CCAGCAACCCAGCATCCAG |
| TDF | 52,911,052 | 52,911,069 | TCTGCCCACCTGACAGTG |
| TDP | 52,911,013 | 52,911,041 | TCTATGGAACCATCTTCTCCATGTACGCA |
| TDR | 52,910,986 | 52,911,007 | CCCAGCGGTGTCTTTAGACTTG |
PCR Primers
| Primer | Sequence |
| SU | GGGTCTCATACCCACTCAACC |
| SD | CCATTTTACCTATTAGCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 292 bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | 372 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10720A-E2 | VGB6 | C57BL/6NTac | |
| 10720B-E11 | VGB6 | C57BL/6NTac | |
| 10720B-D2 | VGB6 | C57BL/6NTac | |
| 10720C-E6 | VGB6 | C57BL/6NTac | |
| 10720C-G4 | VGB6 | C57BL/6NTac | |
| 10720D-H2 | VGB6 | C57BL/6NTac | |
