| Gene | Fpr2 |
| Chromosome | 17 |
| Deletion Start | 17,892,747 |
| Deletion End | 17,893,799 |
| Deletion Size | 1,053 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,893,064 | 17,893,088 | AAACCTGTTTGGAAGTGTCTTCTTG |
| TUP | 17,893,090 | 17,893,112 | TTGCTCTCATTGCCTTGGACCGC |
| TUR | 17,893,115 | 17,893,139 | CCAGACTGGATGCAGAACACAAATG |
| TDF | 17,893,650 | 17,893,671 | GTTTTCATGGGCCAGGACTTTC |
| TDP | 17,893,688 | 17,893,716 | TTCCCTGCCTTATAGTCTTGAGAGAGCCC |
| TDR | 17,893,734 | 17,893,757 | ACTGGTGCTTGAATCACTGGTTTG |
PCR Primers
| Primer | Sequence |
| SU | CACTGTTGAGACATGAGGGC |
| SD | CTTGAGAATATGCATTGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10725E-E12 | VGB6 | C57BL/6NTac | |
